spp error when call peaks
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6.1 years ago
sw ▴ 10

Hello there

Today when I run spp to call peaks, some worked, but some were not. It raised error like this:

Error in data.frame(..., check.names = FALSE) : 
  arguments imply differing number of rows: 1, 0
Calls: find.binding.positions ... calculate.enrichment.estimates -> lapply -> FUN -> cbind -> cbind -> data.frame
In addition: There were 50 or more warnings (use warnings() to see the first 50)

then stopped working. My script is:

Rscript --max-ppsize=500000 run_spp.R -c=sample.bam -i=ctr.bam -p=16 -npeak=300000 -savp -odir=./ -savn=sample.narrowPeak -savr -rf 2>sample_spp.log

by the way, it is not because of high duplication in bam file.

I have no idea how to solve the problem. Any suggestion may help. Thanks in advance.

ChIP-Seq • 1.0k views
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Please use the formatting bar to indicate code. I've done it for you this time.

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Thank you very much. I'll do that next time.

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