Can I compute Tumour Mutational Burden from rnaseq fastq files?

Question

[Pin.Bioinf]

Hello,

I am not familiar with tumour mutational burden, but I was asked to obtain it from fastq files sequenced with RNA-Seq. How can I do it with this data? I think it would be better with exome sequencing though.

Thank you

Answer 1

[Nicolas Rosewick]

check this post : Using RNA-Seq to identify non-synonymous mutational load

[Pin.Bioinf]

Thanks a lot! Actually I saw that post but did not know mutational load was similar to mutational burden. Thanks