DESeq2:Why is there an oblique line in my MA picture, which happened before, what caused this?

Question

[xuyao15927402897]

Why is there an oblique line in my MA picture, which happened before, what caused this? My sample is RNA-Seq data from human lung tissue, 3 control groups, 12 experimental groups clustering

MA plot

[afli]

Could you upload a small set of the count data, and paste your code here? so we can reproduce your result and see what is the cause. Or you can post this on bioconductor https://support.bioconductor.org, Michael Love is very kind and active there.

Aifu.

[russhh]

are there differences in the library-sizes between your treatment-groups?

Answer 1

[xuyao15927402897]

Thank you! I listened to the afli's suggestion and found the answer on Bioconductor:

The answer is here