Hi, community,
I have Hiseq metagenomic data. I have used MEGAHIT assembly the reads, then I binned the contigs which results to 500 bins. I have used CheckM to assess the genome bins. All bins with <10% contamination and >70% completeness were saved and BLASTN to SILVA 132 SSU database. Most bins can detect 16S genes. However, some bins got hit to different taxonomy, such as one contig got hit with 'FUBE01000005.37491.40675 Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia-Shigella;Shigella sonnei', one contig in the bin got hit to '> CP016073.2513631.2516362 Bacteria;Firmicutes;Bacilli;Bacillales;Stapyhlococcaceae;Staphylococcus;Staphylococcus pseudintermedius'. May I know what is the reason? Anything wrong with me?