Extracting miRNA data from RNA-Seq data set

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6.0 years ago

hafiz.talhamalik ▴ 350

Can i extract miRNA data from RNA-Seq data set ? and if possible, can i extract deferentially expressed microRNA's from it ?

RNA-Seq rna-seq miRNA • 1.6k views

ADD COMMENT • link 6.0 years ago by hafiz.talhamalik ▴ 350

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Probably not. Typical RNA-seq library preparation includes a size selection for RNA/cDNA of like 200bp. As short RNAs are (guess what) shorter, whatever signals you pick up will probably be unreliable.

ADD REPLY • link 6.0 years ago by ATpoint 85k

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Ok thanks got it...!

ADD REPLY • link 6.0 years ago by hafiz.talhamalik ▴ 350

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Brother #Hafiz.Talhamalik, If you have extracted the miRNAs from RNA-seq data, please mention here your pipeline, I will be really thankful.

ADD REPLY • link 4.0 years ago by abdul.suboor123 • 0

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no I didn't. as mentioned earlier RNA-seq library is of 150/200bp size so miRNA can't be captured by this as.

ADD REPLY • link 4.0 years ago by hafiz.talhamalik ▴ 350

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