How do I can obtain the promoter sequences of all genes of pseudomonas aeruginosa ?

Question

[saadleeshehreen]

I am looking for all promoter sequences of pseudomonas aeruginosa genes to compare them with the promoter sequence of my gene of interest. Is there any database containing promoter sequences of all known pseudomonas aeruginosa genes ?

Answer 1

[slewenza]

You can download the intergenic regions from the pseudomonas.com site, for any strain.

Many of the intergenic regions are too short to be promoters, but anything over 100 bp is probably long enough to have a promoter.

Sort them and keep all intergenic regions over 100bp, then blast your sequence against these.

This does not include any upstream promoter elements are not within intergenic regions, but are within adjacent coding sequences.