Comprehensive Article To Learn All About In Silico Est Analysis

Question

[Sivabala]

Any concise and comprehensive review article or original research article to learn all about insilico ESTs analysis?

we have uploaded nearly 5000 ESTs in dbEST, now we want to do bioinformatics analysis on that. I found difficulties to collect the papers describe in appropriate manner? Could anyone suggest few?

Thank you in advance.

[Larry_Parnell]

You are much more likely to see more informative answers - and in a timely manner - if you can provide a few details about your interest. What is it that you hope to learn from this EST collection? Were the ESTs generated from a single library or from several that were prepared under different biological conditions?

In other words, are you analyzing the ESTs for gene structure and types of genes present in your organism? Or, are you analyzing these data like a RNA-Seq, gene expression experiment?

Answer 1

[Eric Fournier]

Those two articles by Nagaraj et al could be used as starting points:

A hitchhiker's guide to expressed sequence tag (EST) analysis

ESTExplorer: an expressed sequence tag (EST) assembly and annotation platform

Answer 2

[Woa]

There are several book chapters from Springer:

1. Expressed sequence tags: analysis and annotation

2. EST analysis pipeline: use of distributed computing resources

3. EST databases and web tools for EST projects

4. EST processing: from trace to sequence

[Sivabala]

Thank you very much for your valuable suggestion

Answer 3

[Anuraj Nayarisseri]

Few EST Data Analysis tools are here. Hope it will help you

http://mips.helmholtz-muenchen.de/proj/est/