Usually, adjacent tissue is used as RNA-Seq normal control. However, Adjacent tissue is not accessible in some case. Can I use remove, but same type, tissue instead?
Beside, adjencent tissue in many cases is not typical 'normal' tissue. It may contain certain degree of tumor cells and immune cells which may bring bias to differential expression analysis
Thanks for the comments. I am aware of batch effect in RNA-Seq analysis. We will extract samples in the same batch.
My concern is the use of rmote normal tissue as control instead of adjencent tissue which is not accessible for some reason. I imagine that would be all right since the same tissue would have similar expression profile even it is remote from the tumor. What do you think of that?
I would generally trust samples obtained from adjacent tissue because: