I try to work with CÓNTRA for detecting CNVs and when i try to run the baseline it daesnñt work the error tells me python baseline.py --target /Users/enriquesevillaromero/Bedfile_1/bed_info.modified_autosomas_no_dots.bed --files /Users/enriquesevillaromero/DATOS/covered_N_T_pool/normal_pool/normal_pool1/.bam --output /Users/enriquesevillaromero/DATOS/CONTRA/Baseline/SampleBaseline/ --name baseline_test_normal_pool1 ------ baseline.py ------- i introduce the bedfile and the path with my control sample in .bam the first error tells me WARNING: Genome (-g) files are ignored when BAM input is provided. **WARNING: Genome (-g) files are ignored when BAM input is provided. WARNING: Genome (-g) files are ignored when BAM input is provided. WARNING: Genome (-g) files are ignored when BAM input is provided.
['unionBedGraphs', '-header', '-i', '/Users/enriquesevillaromero/DATOS/CONTRA/Baseline/SampleBaseline/buf/EGAR00001545921_17311.sort.nd.covered.bam.BEDGRAPH.TARGETONLY', and finaly Assertion failed: (!queue.empty()), function ConsumeNextCoordinate, file unionBedGraphs.cpp, line 99. /Users/enriquesevillaromero/Programas/bedtools2/bin/unionBedGraphs: line 2: 19398 Abort trap: 6 ${0%/}/bedtools unionbedg "$@" Traceback (most recent call last): File "baseline.py", line 202, in <module> nSamples_exclude = int(math.floor(TRIMnSamples)) TypeError: unsupported operand type(s) for *: 'float' and 'NoneType'
anybody can help me to find the correct sentence thanks
Hi sevillaro1970, were you able to figure this problem out? When I'm running baseline.py it stops at a similar point: ['unionBedGraphs', '-header', '-i', ...]