Can anybody explain me why this volcano plot is not opening

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6.0 years ago

Beginner • 0

The plot doesnt seem to look like any other. It is not in line either when I draw a scatter plot. Im new to this.. can anybody tell me why the data are not drawing the opening of the volcano by log2 at 0?

volcano

RNA-Seq software error rna-seq • 1.9k views

ADD COMMENT • link updated 5.9 years ago by Biostar 20 • written 6.0 years ago by Beginner • 0

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looks like you've got a consistently higher signal in one set of samples than the other. How did you normalise the (microarray?) dataset?

ADD REPLY • link 6.0 years ago by russhh 5.7k

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Hi Beginner ,

Welcome to Biostars. I have cleaned up this discussion and changed your post. You had put the explanation of your question as image description, and as such it was invisible to us. Note that you can at all times edit your own post and provide more information.

Could you elaborate on which steps were taken prior to generating this volcano plot?

ADD REPLY • link 6.0 years ago by WouterDeCoster 47k

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