bowtie2-align died with signal 6 (ABRT) (core dumped)

Question

[Ric]

I do not know why I got the below error:

bowtie2 -x tdna -U s21.fq -S sample5.sam 
Error: reads file does not look like a FASTQ file
terminate called after throwing an instance of 'int'
(ERR): bowtie2-align died with signal 6 (ABRT) (core dumped)

Thank you in advance.

[Jeffin Rockey]

Please share first few lines of s21.fq

[Jeffin Rockey]

Also, are you using some very old version of bowtie2. Please specify the bowtie2 version.

[lakhujanivijay]

Check validity of your fastq files

Checking fastq is valid

[Ric]

Thank you all, my fastq file was broken.

[lakhujanivijay]

How did you discover that ?

[Ric]

I opened the file and saw that accidentally during the trimming stage I converted it to a FASTA file.