Entering edit mode
6.0 years ago
shibl_a
▴
20
I installed checkm using bioconda and downloaded pplacer from its site. I then moved all the pplacer executables into the checkm bin.
When I run this command:
$ checkm lineage_wf --pplacer_threads 2 checkm_input checkm_output
I get this output:
*******************************************************************************
[CheckM - tree] Placing bins in reference genome tree.
*******************************************************************************
Identifying marker genes in 10 bins with 1 threads:
Finished processing 10 of 10 (100.00%) bins.
Saving HMM info to file.
Calculating genome statistics for 10 bins with 1 threads:
Finished processing 10 of 10 (100.00%) bins.
Extracting marker genes to align.
Parsing HMM hits to marker genes:
Finished parsing hits for 10 of 10 (100.00%) bins.
Extracting 43 HMMs with 1 threads:
Finished extracting 43 of 43 (100.00%) HMMs.
Aligning 43 marker genes with 1 threads:
Finished aligning 43 of 43 (100.00%) marker genes.
[Error] Make sure pplacer is on your system path.
Controlled exit resulting from an unrecoverable error or warning.
Please note that when I run this:
$ which pplacer
I get this location, where all checkm executables are:
~/miniconda3/envs/checkm2/bin/pplacer
Any ideas/ suggestions?
Why didn't you install pplacer from bioconda as well? Could you try installing both from bioconda and give it a try?
That was the first thing I tried but this is what the conda command returns