Basic Bioinformatics Analysis Of Shrna

Question

[Pfs]

Any pointer (tutorial, review, pipeline examples) on the steps required for the bioinformatic analysis of shRNA data?

Thanks in advance.

[Ying W]

what do you mean by shRNA data? what kind of analysis? are you interested in expression changes before and after shRNA knockdown?

[Michael]

shRNA is an engineered contruct, I don't think there is anything like large scale data except its sequence and the secondary structure, which are both known by design. maybe you mixed it up with one of the xyzRNA types.

[Pfs]

Yes, I would be interested in expression changes.

Answer 1

[Chris Evelo]

It is commercial. But there is a lot of information in the learning center here: http://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna.html