The evidence of the gene annotation

Question

[amalsalman91]

Hello

I am working in annotation project How could I make a evidences for the annotation . is it from the domain that show in mRNA blastx Or from the protein sequences that coming from mRNA by ORF finder

[Tm]

According to me, both can work as evidence. But in the case of blastx result, those are only annotated sequences which you consider, on the other hand latter gives you a complete set of proteins.