Entering edit mode
6.6 years ago
arunprasanna83
▴
60
I am trying to use pacbio long reads to do gap filling using GapFiller program. What is the correct way of specifying the insert size mentioned in library file ? In other words, how to estimate insert size for pacbio long reads (SMRT).
Thanks in Advance.
You should probably use PBJelly (https://sourceforge.net/p/pb-jelly/wiki/Home/). Here are some instructions for running PBJelly for PacBio Sequel subreads and error correcting using a variant caller instead of Pilon to correct indels (