kallisto with reference genome
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5.9 years ago
vm.higareda ▴ 30

Is it correct to use a reference genome to build a kallisto index and use this index to run kallisto quant?.

I have genome of a bacteria, extracted the complete sequence of the genes and used this multi fasta file to build the index

RNA-Seq kallisto • 2.3k views
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I have genome of a bacteria, extracted the complete sequence of the genes and used this multi fasta file to build the index

What you did is correct, but it is not a "reference genome", you may call it reference gene set or simply reference transcriptome.

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