Is GC-bias correction valid for MeDIP-seq data preprocessing?

Question

[tofukaj]

Dear all great helpers,

Practically, I'm a biologist and I've just start learning MeDIP-seq analysis. Due to my lack of experience in both concept and data pre-procession, I'm wondering if the performance of GC-bias correction is valid with such data. As far as I understand MeDIP aim to enrich methylated DNAs, CpG regions were highly expected. However, I'm wondering if both low and high CpG regions are supposed to be included?

I must apologize you in advance if I've totally mistaken anything in my question.

Best regards Kaj

Answer 1

[biostart]

MeDIP enriches for high-CpG regions by the design of this method. So if you have in mind a GC correction as in ChIP-seq, then no, it would not be applicable to MeDIP.