Question

coordinates of H3K27Ac

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5.9 years ago

rajesh ▴ 60

Hello everyone H3K27Ac modification marks for active transcriptional region. This also marks for regulatory region activation. Is there any resoiurce or site, where these H3K27Ac coordinates are listed.

Thanks in advance.

ChIP-Seq • 1.2k views

ADD COMMENT • link updated 5.9 years ago by asntech ▴ 50 • written 5.9 years ago by rajesh ▴ 60

score 2 · Answer 1 · 2019-01-06

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5.9 years ago

asntech ▴ 50

Apart from ENCODE, you can also use Cistrome DB http://cistrome.org/db/ to search and download H3K27Ac peaks (in BED format) and signal tracks (bigWig format). If you're interested in regulatory regions, SCREEN has millions of candidate cis-regulatory regions defined by the combination of several histone marks and other factors in mouse and human genomes http://screen.encodeproject.org/

ADD COMMENT • link 5.9 years ago by asntech ▴ 50

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Thank u very much...these link contain enough information.

ADD REPLY • link 5.9 years ago by rajesh ▴ 60

score 1 · Answer 2 · 2019-01-04

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5.9 years ago

ATpoint 85k

H3K27ac is highly cell-type specific. Therefore, you'll first need to define which cell type you want to look at. ENCODE harbours plenty of ChIP-seq data for this histone mark, UCSC has layered tracks for it available and if none of this fits, search NCBI for published datasets from which you can retrieve published scores and coordinates (if the authors provided it) or download the raw data and map them yourself.