interpreting local blast output

Question

[manjumoorthy95]

Can anyone tell me how to understand or interpret the result from localblast , in the tab delimited format? I am getting result like this :

NODE_1_length_1733100_cov_8.771358  NZ_JMSZ01000042.1   75.07   2230    508 41  85965   88170  275749   273544  0.0 994
NODE_1_length_1733100_cov_8.771358  NZ_JMSZ01000042.1   76.23   345 80  2   85391   85734   276296  275953  9e-43   182

can anyone tell me what each column mean?

Answer 1

[Joe]

You can find this information in google easily enough:

https://edwards.sdsu.edu/research/blast-output-8/

This assumes you used output format 8 and left all settings as defaults. You should edit your question with the command you used to generate that output though else we’re just stabbing in the dark really.

query  | subject  |  %id  |  alignment  |  mismatches  |  openings  |  query  |  query  |  subject  |  subject  | E-value  |  bitscore
       |          |       |   length    |              |            |  start  |   end   |   start   |    end    |          |

[manjumoorthy95]

thank you for the interpretation