I am trying to sequence a random library reporter midiprep (hence a mixture of nucleotide at region inside the random sequence). I have heard that ab1 Peak Reporter is a great tool by ThermoFisher. However, so far, the software seems to be disconnected for me (can't find the link to download it).
Is there any alternative approach to this problem (I tried QSV analyzer but couldn't fix a bug due to my window virtual machine).
Thanks!
It may be available by creating an account at this link. Original source link for
ab1 peak reporter
is in this paper.If you only need a peak viewer then check out CutePeaks.
Thanks for the response! So I created an account at the link, and seems like the only thing that is close to ab1 peak reporter is Variant Analysis (which is also intuitive to use for me). The link in the paper is no longer functional.
Perhaps that tool is depreciated. If you were able to use the Variant analysis then that is great.
We do have ab1 variant calling in Indigo https://www.gear-genomics.com/indigo/ maybe that solves your problem?
The ab1 Peak reporter tool is no longer available as singular application. However all the functionality of the tool has been transferred and embedded into the cloud-based "Thermo Fisher Connect your Lab" suite of apps for Sanger sequencing analysis, notably the "QC" app for quality control (equivalent to the Applied Biosystems Sequence scanner program), the "VA" app for variant analysis (mutation detection) and "NGC" app (next generation confirmation). These apps are free of charge. After importing ab1 files into a project using any of the apps named above they can be selected in the "Traces" tab and exported via button "Actions" -> "Export Traces" -> " *.peaks.csv " The resulting spreadsheet will look exactly as the output of the ab1 peak Reporter tool.