Can I Search Pride On Drugs?

Question

[Egon Willighagen]

I tried searching paracetamol in PRIDE, to see the differential effect on the proteome when a cell or organism is dosed with a drug, but could not find anything. Browsing PRIDE shows that it has room for species, tissue, cell type, GO term, and disease, but not a word about small molecules, like drugs, hormones, or other chemical entities of biological interest...

Does PRIDE contain proteomics for drug/metabolite perturbation studies? And if so, how can I search based on a drug/metabolite name?

Update: OK, I am educated by @prideebi and @ChrisEvelo now: PRIDE is a protein/proteome database, more than a proteomics database... like the HMDB is a metabolome rather than a metabolomics database.

Update2: @pride_ebi further details that 'separation' dimensions and intensities are present, and suggest searching via PubMed abstracts for useful differential protein abundances, and use the PubMed ID to search PRIDE.

[Jeremy Leipzig]

Best Phrased Question of the Day

[Torst]

I believe Crick may have experimented with LSD to help come up with the structure of DNA, so maybe it will help you search PRIDE too.

Answer 1

[Chris Evelo]

You might first want to check the answer to this question.

It indicates that pride is really just about protein identification. The terms you mention that are searchable in Pride are also useful for proteins themselves.

Now I am not sure what kind of perturbation you are looking for. If the protein itself changes, for instance by drug protein adduct formation you would in fact see that from the spectrum and the change might be relevant for the spectra stored in Pride. But... Most often the change will affect protein levels and as mentioned in that other question these are not stored in Pride.

Edit

There were a number of updates from people on Twitter that knew about the most recent developments at Pride. It turns out that Pride now actually does store protein concentration information, at least for a number of studies.

PRIDE is also storing protein expression information now. http://www.ebi.ac.uk/pride/q.do?accession=16649 (use PRIDE Inspector). You can also check out 16649 - 16656. (@pride_ebi and @juan_vizcaino)

[Egon Willighagen]

Ah, OK. I had indeed not realize that PRIDE content was decoupled from whole-sample analysis... proteomics database vs protein database... thanx for the clarification!