Question

Multiplexed 454 Data Analysis

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13.1 years ago

User 3466 ▴ 10

I'm a newbie and I'd like to know how to start doing analysis of multiplexed 454 data. I know its wide question but I really don't know where to start?

next-gen sequencing • 3.1k views

ADD COMMENT • link updated 13.1 years ago by Johnny Brown ▴ 140 • written 13.1 years ago by User 3466 ▴ 10

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Do, or do not. There is no try. :)

ADD REPLY • link 13.1 years ago by Neilfws 49k

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Your sequencing provider should demultiplex the data for you, assuming you used the standard Roche MID barcode system.

ADD REPLY • link 13.1 years ago by Torst ▴ 980

score 2 · Answer 1 · 2011-10-23

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13.1 years ago

Istvan Albert 102k

check out the mothur package, it has 454 flow trimming and demultiplexing features that are very handy and usable even if your data has nothing to do with metagenomics

ADD COMMENT • link 13.1 years ago by Istvan Albert 102k

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thanks i will try with that

ADD REPLY • link 13.1 years ago by User 3466 ▴ 10

score 0 · Answer 2 · 2012-01-30

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12.8 years ago

Johnny Brown ▴ 140

Qiime may be useful to you. Particularly handy is the MacQiime all-in-one install for apple computers.

ADD COMMENT • link 12.8 years ago by Johnny Brown ▴ 140