Reciprocal blast hit

Question

[BioBaby]

hello, I tried to find out homologous between two species for that I did reciprocal blast means A to B and B to A. so now I having two blast output in tabular format but now I do not how to find RBH.I searched for script but could not find suitable for my data. please help me..

Answer 1

[colindaven]

I like using proteinortho for this task. It will give you a single table containing reciprocal blast hits and just requires one command to run.

https://www.bioinf.uni-leipzig.de/Software/proteinortho/

[BioBaby]

Thanks @colindaven for your positive response. I used OMA https://omabrowser.org/standalone/