Specific Primer Design for a new organism

Question

[firminlago]

I am a newbie in the field so pardon my "unknown" I a have a whole genome sequenced of a lactobacillus I want to design primer specific for it. what will be a good region to go after and how do i go by finding the region? how do i design the primer and test it to make sure it is specific? Thanks much for answering or direst me to the correct tuts.

[natasha.sernova]

There is a general guide in NCBI how to do it :

I took the link above from thefollowing site: https://www.researchgate.net/post/How_to_select_primer_for_genus_identification_of_lactobacilli_and_lactococci