rna seq single ended -Information on Rseqc report

Question

[ASid]

I am newbie for ngs data analysis and so my questions would be very naive. With a lot of struugle I could use infer_experiment.py of rseqc to infer the stranded ness of the single ended publicly availaible dataset data that I was using. The output is displayed as follows:

This is SingleEnd Data Fraction of reads failed to determine:0.0198 
Fraction of reads explained by "++,--": 0.4819 
Fraction of reads explained by "+-,-+": 0.4982

So can anybody help me in interpreting it that it is stranded or unstranded and how we would guess that?

1. I use hisat2 for alignment and there was an option about strandedness and I used unstranded so it should be fine righht ?

Answer 1

[lshepard]

This is unstranded RNAseq. You can tell by the almost equal distribution of reads explained by forward and reverse output.