I added the T. aestivum snpEff4.3 genome database in Galaxy (https://usegalaxy.org/?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2FsnpEff%2F4.3%2BT.galaxy1&version=4.3%2BT.galaxy1&__identifer=rjgm18erx4), seems the database has been added as I could see theT. aestivum snpEff4.3 genome database at the right side history of Galaxy page column. But, when I add VCF file and snpEff4.3 wheat database, it's showing error. I would like to annotate the natural variants against the wheat genome (particularly against wheat homeolog 3DL). I also tried with VEP ENSEMBL, it is working, but I would like to try with Galaxy to see the annotations from Galaxy. Does anyone know about this?
You should post this question over at Galaxy help site.