EdgeR differential expression and heatmap
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5.8 years ago
Kasthuri ▴ 300

I performed a differential gene expression analysis for the red vs. blue group as shown in this image below (RNA-seq data) using TCGAbiolinks which uses the EdgeR package. Clearly, there is a difference between the groups however the difference is due to under-expression in some samples in the blue group than overall difference uniformly across the groups. How do we generate a heatmap that shows differential expression for situations like these? I could average each group for each gene separately and plot, but is that legitimate? Thanks.

Here is the link, if it is not properly hyperlinked: DGE

RNA-Seq • 1.8k views
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Please see How to add images to a Biostars post, I did it for you this time.

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Thanks, I figured out.

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