Identification of Long Non coding RNa in De novo

Question

[AyehSadr]

Dear All

I am working on Identification of long non-coding RNAs in scorpion, in this case the genome reference is not available, I have to do blast for to all transcript candidates and I don't find the closest genome. I would appreciate to guide me about the best the best approach.

Best Regards Ayeh

[Nicolas Rosewick]

Which type of data do you have ? RNA-Seq ?

[AyehSadr]

Yes, 150 PE and transcriptome assembly process by means of Trinity

Answer 1

[Nicolas Rosewick]

Could you edit your question with these informations. You could try trinotate : https://github.com/Trinotate/Trinotate.github.io/wiki