How to merge read count of multiple sample in one fiel to be used for DESeq2 fold change study?
1
0
Entering edit mode
5.9 years ago
Neu ▴ 10

Hi, I have read count of circRNA junctions obtained by analyzing RNA-Seq data. How can I merge these files to calculate fold change using DeSeq2?Some sample may contain junctions that are not detected in other sample or replicate analysis and I need to put zero in that condition. Any help will be appreciated. .

alignment sequencing next-gen RNA-Seq • 2.3k views
ADD COMMENT
1
Entering edit mode

Have you tried full_join() from dplyr?

ADD REPLY
0
Entering edit mode

DESeq2 has several features for importing data: did you give a look to them? You can find them here

ADD REPLY
0
Entering edit mode
5.8 years ago
gayatri281 • 0

Hi Neu, Did you figure this out? I am looking for a solution to the same problem!! Thank you!

ADD COMMENT
0
Entering edit mode

Hi, I used ablebits-ultimate-suite-excel add-in to do this. Before doing this, I made a single table for the identical columns by merging all the sample result files and then sorted them.

ADD REPLY

Login before adding your answer.

Traffic: 1667 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6