How to create local database for doing BLAST

Question

[Mehar Imran Zafar]

hello everyone please guide me about how I can create a local database for identified genes by BLAST search against the EST sequence

how I can download EST Sequence of the respective plant species(eg Oryza sativa) in the local database and how I can perform BLAST against this EST sequence via identified genes

[Bastien Hervé]

You can take a look at E-utilities

[Benn]

Or follow some tutorials which can be found on google, I found for example here one.

Answer 1

[GenoMax]

Information you need to create local blast databases is available in this NCBI blast handbook.