IGB won't open .gff file.
0
1
Entering edit mode
5.8 years ago
sj93 ▴ 10

The IGB website says their program supports many different file formats, including gff. I have saved a genome from NCBI in several different file formats to my computer (using Ubuntu), but when I go to open the custom genome from IGB, it only recognizes the .fna file type, when I have genomes saved as .gbff and .gff in the same folder. It's like the program won't recognize these file types saved on my computer. Does anyone know why this might be happening? Thanks in advance.

IGB gff sequencing genome gff3 • 2.0k views
ADD COMMENT
0
Entering edit mode

Could you provide a sample of your gff fille ? Do you load the fasta separately or is it embedded in your gff ?

ADD REPLY
0
Entering edit mode

The fasta is not embedded, I tried it separately for comparison. I'm not sure what the best way to provide a link to my gff file would be, but the file is available on the top of the page at this location https://www.ncbi.nlm.nih.gov/genome/24088.

ADD REPLY
1
Entering edit mode

The GFF contains the annotation. It can sometimes contain the "genome sequence" using embedded FASTA that Juke-34 refers to, but more often the genome sequence is in a FASTA file (extension .fa, .fasta, or .fna sometimes from NCBI). You can download the FASTA from NCBI too and probably will open that first and then the annotation as GFF

ADD REPLY
0
Entering edit mode

Thank you very much! :)

ADD REPLY
0
Entering edit mode

This gff file is completely stupid, never seen that before. It doesn't contain anything interesting, no structural annotation (position of feature like gene, trna, pseudogene, etc). It looks like description of sequences of an assembly. It describes the size of 80 000 sequences. So it's a gff of an assembly... I'm wondering how useful that could be ???

So according to this gff file there is nothing to display, so it's normal you don't see anything in IGB.

ADD REPLY
0
Entering edit mode

This is a mitochondrial genome (not a full genome) AFAI see.

ADD REPLY
0
Entering edit mode

She/he said at the top of the page...

Anguilla rostrata (American eel) Representative genome: Anguilla rostrata (assembly ASM160608v1) Download sequences in FASTA format for genome Download genome annotation in GFF, GenBank format BLAST against Anguilla rostrata genome

I don’t think she/he talks about the mitochondrial genome.

ADD REPLY

Login before adding your answer.

Traffic: 2748 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6