Question

How to convert paired end data to single end?

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5.8 years ago

archana.bioinfo87 ▴ 210

Hi,

Can anyone help me out to find a tool or software to convert paired-end data to single-end. I have more than 10k paired end data.

Thanks

RNA-Seq • 2.2k views

ADD COMMENT • link updated 5.8 years ago by GenoMax 147k • written 5.8 years ago by archana.bioinfo87 ▴ 210

score 0 · Answer 1 · 2019-02-26

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5.8 years ago

GenoMax 147k

If your data files are interleaved

@Frag1_Read1
@Frag1_Read2
@Frag2_Read1
@Frag2_Read2

then use reformat.sh from BBMap suite.

reformat.sh in=interleaved_fq.gz out1=R1.fq.gz out2=R2.fq.gz int=t

ADD COMMENT • link 5.8 years ago by GenoMax 147k

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Thanks any other tool as well.

ADD REPLY • link 5.8 years ago by archana.bioinfo87 ▴ 210

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What?

ADD REPLY • link 5.8 years ago by WouterDeCoster 47k