Cross assembly of metagenomics

Question

[MAPK]

I am working on metatranscriptomic data and wanted to use this tool available here: https://edwards.sdsu.edu/crass/# I have assembled my transcriptomic data using Trinity and have got Trinity.fasta file. However, this tool also requires ace format file, which I don't have. What do I need to do to get ACE file or specifically how do I run this program? I would really appreciate if someone clarifies.

[5heikki]

This tool is meant for situations where you have multiple assemblies, e.g. 10 gut microbiota assemblies from different people..

Answer 1

[onestop_data]

CrAss was designed for comparative metagenomics. You assemble all your metagenomes together, and the tool leverages the overlapping across samples to infer the distance within samples.

The tool first required an ACE file, but this not common anymore (I think MIRA assembler generated it as one of its outputs). Luckily it accepts SAM file (*) (not BAM) to replace the ACE file.

Here is a tutorial on how to run the tool step-by-step

(*) the SAM file reflects the maps all samples reads against the cross-assembly (contigs)