Error when running sma3s for proteome annotation

Question

[l.souza]

Hello everyone,

I have been trying to run sma3s for protein annotation, according to the author's instructions (UPOBioinfo Group). However, when running the command

./sma3s.pl -i query_dataset.fasta -d uniref90.fasta -goslim

I got the message "Problem with blastdbcmd. It could be due to an empty or erroneous Blast report file, indexed fasta, or low RAM memory". When I check the files in the folder, the uniref90.fasta file has been emptied.

Does anyone know how to solve it?

Thanks in regard,

Lucas

[Neel]

Hi, I am facing the same problem. Have you found a solution to it? If you know then plz let me know.