Hi
I would like to create a PCA plot on samples to see how 15 samples in my data group based on the methylation profile of common methylated fragments ? I have data for 400,000 common methylation fragments for each sample. I have transposed the file now in such a way that there are 15 rows representing samples names and 400000 columns representing methylated fragments, in my csv file. I tried using some functions in R such as FactoMiner but I was unable to create any plot. Does any of you have any suggestion on how can I create a PCA plot with transposed data file I have in .csv format?
Any help will be greatly appreciated. Thanks.
What exactly did you try and did you get an error?