How to split Fastq file into Forward, reverse fastq file.
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5.6 years ago
9521ljh ▴ 50

Hi. i have SRR0000.sra file.

and i have SRR0000.fastq using SRA toolkit.

then i want to split it forward fastq and reverse fastq. how to????

i try to use SRA toolkit - fastq-dump -I --split-files SRR0000.sra

but the output is just the half of file. i don't think it is right for forward and reverse.

gene sequencing sequence genome • 2.9k views
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this issue has been mentioned before, even here on biostars:

How to split paired end SRA file into 2 correct fastq files

in other words, have you tried looking for an answer before posting your question?

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