How Do You Find Insertions And Deletions In A Dot Plot ?
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13.0 years ago
User 5037 ▴ 290

Hi .When ever there is a match a dot is plotted in a dot plot. When there is no match no dot. Then does it mean that where ever there are no dots that a insertion or deletion has taken place?

How do you discover on which sequence the insertion or deletion has taken place ?

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13.0 years ago

It's more complicated than just looking at dots/no dots. You also have to take into account the pattern of continuous diagonal dots. Having no dots can mean a substitution. Usually a shift of the diagonal indicates a deletion or insertion.

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shift to the right means deletions/insertions on the sequence on top right ?

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12.9 years ago

First, you need to tell us if this is seq A dot-plotted against seq A' or against seq B, where A' is the version very much like A but with suspected insertions/deletions, but B is simply a much more distantly related sequence.

Second, one needs to know the window and threshold for plotting the dot. If the window = 1 and the threshold = 1, then perhaps a dot = a match and no dot = in/del - or no dot = a mismatch. Normally the window is much greater and the threshold is less than 100% (match).

Thus, as DK writes (+1), it is more complicated than dot/no dots. As I often tell people, many such bioinformatics activities are easy. So, just set up a series of A type sequences, where you have seq A, A + insertion, A - deletion, A + A, rev complement of A and so on. Then, make the dotplots of all these against A to see what they look like. Once you've done that, you'll have your own dotplot glossary, but more importantly, you'll have taught yourself much more than we can type here and you'll be smarter for it.

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