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5.5 years ago
casley_queiroz
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20
422/5000 Hello. Help me interpret the output of Trininty/edgeR from differential expression analysis. It generates two files, one with UP-expresssion for SampleA and one with UP-expression for SampleB.
I know that logFC positive means UP-expression and logFC negation down-expression. Why in the output of UP-expressed from SampleB is the logFC negative? Can someone help me to interpret this data?
the summary of the sample outputs:
sampleA UP-expression
enter code here sampleA sampleB logFC logCPM PValue FDR diseased healthy
enter code here TRINITY_DN20_c0_g1_i1 diseased healthy 11.547219358876 6.46292409746543 9.85640289124254e-24 2.15436326195334e-19 219.127 0.069
enter code here TRINITY_DN2989_c0_g1_i11 diseased healthy 14.4550438923786 5.66470792550252 4.15351643702967e-22 4.5392742761188e-18 162.136 0.000
enter code here TRINITY_DN6792_c0_g2_i10 diseased healthy 14.2844065813709 5.49525087238527 1.34541228845186e-21 8.87815749571852e-18 77.118 0.000
sampleB UP-expression
enter code here sampleA sampleB logFC logCPM PValue FDR diseased healthy
enter code here TRINITY_DN325_c0_g1_i7 diseased healthy -13.0824601986213 7.08358351895648 1.96212428498806e-27 1.71548526236506e-22 0.000 46.006
enter code here TRINITY_DN325_c0_g1_i1 diseased healthy -12.1211236235678 6.12036009218274 1.51458619514056e-24 6.62101355205698e-20 0.000 23.937
enter code here TRINITY_DN7303_c0_g1_i6 diseased healthy -14.6691392658181 5.86419527682427 8.82661700411335e-24 2.15436326195334e-19 0.000 45.195
Help me
If m not getting it wrong. SampleA Up-expression means up regulated in A sample and down regulated in B sample. and other file means Up in sampleB and down regulated in sampleA