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5.5 years ago
giu.lat82
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0
Hi I am analyzing RIP Seq experiments. I downloaded several RIPSeq experiments where the authors performed immunoprecipitation for PIWIL1 and then sequenced the small RNAs part. After performed the detection of piRNAs and other classes of small RNAs i found a huge number of reads mapping on genes (mRNAs and lncRNAs). Could they be target of the identified piRNA? Or are they degradation products? Which strategy could I use to predict piRNA- target interaction using these data?
