Entering edit mode
5.6 years ago
hsu
▴
40
Hi, I want to remove sepecific sequence in the 5' of my fastq file, these sequeces which I want to remove are not adapter sequences. I tried to use
cutadapt -g ATGCATTTTCG -o out.fq my.fq
But it's not working. Do you have any suggestions? Thank you !
Hi hsu,
Could you show us how the read with the desired sequence that needs trimming looks like? Your command works for me:
You can use
bbduk.sh
from BBMap suite this way.