Peak calling using spp package

Question

[chaudharyc61]

Hello everyone I'm using spp package for Chip-seq data so while reading the bam files, the data is showing three elements 1. tags, 2. flen and 3. quality What is flen ?

Answer 1

[Devon Ryan]

Fragment length, which is also known as template length.

[chaudharyc61]

While calling peak it is creating problem I am using spp package for peak calling and it is not giving binding characterstics

[Devon Ryan]

SPP is relatively obscure, with few people outside of ENCODE ever using it. Try a more common peak caller like MACS2 or SICER.