pacbio-illumina hybrid assemblies

Question

[from the mountains]

We are looking into assembly with pacbio and illumina data, and wondering if there are any tools or well-validated workflows for hybrid assemblies, as well as any recommendations for coverage. this is for a eukaryotic genome. additionally, has anyone ever tried a pacbio-10XGenomics hybrid assembly?

[h.mon]

https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/Large-Genome-Assembly-with-PacBio-Long-Reads

Answer 1

[ricardoguerreiro2121]

This guy seems to be trying it with quickmerge.

https://github.com/mahulchak/quickmerge/issues/22

It would be good to use the advantage of the 10x supernova assemblies and not just feed them as simple illumina data to another assembler...