Where to find WGCNA tutorials?

Question

[BioNetwork]

I would like to use the WGCNA package but the tutorials are gone (http://labs.genetics.ucla.edu/horvath/CoexpressionNetwork/Rpackages/WGCNA/) and I cannot find any free tutorial that explains how to execute their main analysis pipelines.

[BioNetwork]

I found this: https://hms-dbmi.github.io/scw/WGCNA.html but it is not comprehensive enough.

[BioNetwork]

I think this is the new link to the old tutorials, could someone confirm? https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Book/

[mxlsherry1992]

Hi, Thank you! But it seems that it need at least 15 samples, if I only have 7 timepoint, 2 replicate for wach time point, if I can still use WGCNA....

Answer 1

[Kevin Blighe]

The new location is here:

• https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/

It seems to have been moved within the last few days (time of writing: August 22, 2018).

Kevin

[mxlsherry1992]

Hi, Thank you! But it seems that it need at least 15 samples, if I only have 7 timepoint, 2 replicate for wach time point, if I can still use WGCNA...

[Kevin Blighe]

With 2 replicates per time-point, your set-up is not great. Why only 2? It will be very difficult to publish that in any reputable journal.

[mxlsherry1992]

Hi, thanks for reply, we didn't aim to do WGCNA at first, but just think about if it could be done or as a reference for results:)