Tool for visualisation of blast results
2
1
Entering edit mode
5.4 years ago
s.m.frampton ▴ 20

Hello,

Is there a tool that provides a nice visualisation of the homology between 3 genes? Furthermore, I also really need to be able to see where the exons are within the figure to see which exons the genes share...

I have three homologous genes from a complex region of the human genome whereby one of them is a fusion of the other two (gene 1). I am trying to learn the technical limitations of short- and long-read sequencing when studying this region so need an understanding of where the reads have originated from and where they are likely to incorrectly map to.

I'm interested in a tool that generates something like the image attached (made with Kablammo) but with some tweaks. Kablammo only creates figures of homology between 2 genes and adjusts the size so they all look the same length, plus information about exons and introns is lost. I have made the attached figure by swapping which gene is the query/subject and attaching my own labels in PowerPoint.

Perhaps within the evolutionary genomics field there is a neat tool that can display all this?

Many thanks in advance! enter image description here

alignment homology RNA-Seq evolutionary genomics • 5.0k views
ADD COMMENT
1
Entering edit mode

maybe CoGe tool GEvo (https://genomevolution.org/wiki/index.php/GEvo ) could work or worth taking a look at.

ADD REPLY
1
Entering edit mode
5.4 years ago
Asaf 10k

Take a look at genoplotR, it might need some R coding though.

ADD COMMENT
1
Entering edit mode
5.4 years ago
Joe 21k

You can take a look at this thread I've been curating Interesting/useful/beautiful bio sequence viz tools

Not all of those suggestions will fit the bill, but EasyFig probably would.

ADD COMMENT

Login before adding your answer.

Traffic: 1599 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6