How to mapping DNA read pairs to reference according to particular gene ?
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5.4 years ago
9521ljh ▴ 50

i have tumor.fasta file and i want to mapping it to reference Only TP53 region

How can i ?
genome next-gen sequencing gene assembly • 786 views
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By getting TP53 region sequence you are interested in as fasta format file. Followed by using blat (http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/blat/ ) to do alignment of your tumor.fasta to the reference.

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What does tumor.fasta contain?

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