Entering edit mode
5.8 years ago
ZheFrench
▴
590
Wondering what to use to start the analysis of a public single cells project.
Libraries were prepared with Smart -Seq2 protocol. Sequencing libraries were generated and indexed from 0.25 ng of cDNA per sample using the Nextera XT DNA Library Preparation Kit.
perhaps not fully up-to-date anymore but still a good starting point :
https://github.com/seandavi/awesome-single-cell
Should be. Last update 10 days back.
HumanCellAtlas Github repo has a collection of single-cell analysis pipelines.
Code: https://github.com/HumanCellAtlas/skylab Wiki: https://github.com/HumanCellAtlas/skylab/wiki/SmartSeq2-Pipeline-(v0.2.0)