Help with EPIC methylation array data analysis - minfi, noob and dmpfinder
0
0
Entering edit mode
5.4 years ago
veronico ▴ 70

I have EPIC data from 8 samples.

Sample sheet: Sorry I am new to posting here, so please forgive my bad formatting:

Plate   Bead chip # Well Position   Well Position   Sentrix_ID  Array   Complete Barcode    Sample_Name
1447    1   A01 01A 201172520042    R01C01  201172520042_R01C01 MCF10A N 2d
1447    1   B01 01B 201172520042    R02C01  201172520042_R02C01 MCF10A H 0.5% 2d
1447    1   C01 01C 201172520042    R03C01  201172520042_R03C01 68 N 2d
1447    1   D01 01D 201172520042    R04C01  201172520042_R04C01 68 H 4% 2d
1447    1   E01 01E 201172520042    R05C01  201172520042_R05C01 68 H 0.5% 2d
1447    1   F01 01F 201172520042    R06C01  201172520042_R06C01 68 N 8 wk
1447    1   G01 01G 201172520042    R07C01  201172520042_R07C01 68 N 3d
1447    1   H01 01H 201172520042    R08C01  201172520042_R08C01 68 H 4% 8wk

I am trying to find DMPs from the samples N 8 wk and H 4% 8wk. I did the following for normalizing and processing:

setwd("~/Desktop/HYPNOR/")

baseDir <- file.path("~/Desktop/HYPNOR/")
list.files(file.path(baseDir, "201172520042"))
list.files(file.path(baseDir))

targets <- read.metharray.sheet(baseDir)

RGset <- read.metharray.exp(targets = targets)
RGset
phenoData <- pData(RGset)
phenoData[,1:6]

pd <- pData(RGset)
pd
pd[,1:4]

qcReport(RGset, sampNames = pd$Sample_Name, pdf = "qcReport.pdf")

#Preprocessing/Normalizing/DYE correction
noob <- preprocessNoob(RGset)
RGset
GMset_map <- mapToGenome(noob)
snp_add <- addSnpInfo(GMset_map)

remove_snps <- dropLociWithSnps(snp_add, snps=c("SBE","CpG"), maf=0.05)

dmpFinder(remove_snps, pheno = pd$Sample_Group, type = c("categorical"))

What I have tried hasn't worked and I don't know how to single those two samples only for dmpfinder. I made a new sample sheet with just the two samples, but that didn't work and also created a subset of the data, and that didn't work.

I was wondering if there is a better solution for this? I am quite new to EPIC methylation analysis, so my apologies if I sound naive.

Any help would be awesome, please. Thank you.

R • 1.4k views
ADD COMMENT

Login before adding your answer.

Traffic: 2109 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6