3 prime RNA sequencing depth fro DEG analyses (Quantseq)

Question

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Hi

Is there any paper were people have analyzed the optimal number of reads for differential expression analyses using quantseq 3' sequencing. So far I've only been able to find papers were authors compared differences between "standard" RNA sequencing vs 3' sequencing, normally starting from 10 million reads and then downsampling to compared the differences.

Is there any optimal number of reads for DEG analyses using 3' RNA sequencing?

Thanks