DEG tool for just 2 pair end data

Question

[sabaghianamir70]

Hi every one

I doing a friend a favor about his project,

He has only 2 dataset, one of them is control and one is treat, those are pair end data and he does not had the budget to send more sample for rna sequencing. So anyway i did align those data and finally i cant find any tool for differential expression, i tried limma edgeR and deseq2, even salmon i tried out.

Is it even possible ? and if it is, suggest me which tool.

P.S i worked with Star and then featurecount

Thanks

[ATpoint]

Please use the search function towards analysis of unreplicated data:

Analyzing RNA-seq without replicates

Advice on RNASeq analysis without biological replicates for differential gene expression.

Analysis Without Replicates

#rna-seq 2 tissue, 2 different individual, no replicate, what can i analysis?

Are 2 replicates per sample sufficient for RNA-seq data analysis?

What shall I do with this RNA-seq data that have no replicates?

Also page 23, section 2.11 of the edgeR manual (https://www.bioconductor.org/packages/release/bioc/vignettes/edgeR/inst/doc/edgeRUsersGuide.pdf) addresses this.