Entering edit mode
5.4 years ago
ste.lu
▴
80
Hi there!
I was wondering if anyone can help me out in a doubt I have about the calculation of the replication time.
One can infer the replication time of the DNA by looking at the DNA amount of a region and compare it to the average of its chromosome. e.g. if I sequence Chromosome 1 and I see double of the amount of a region then I could think of that region as an early 2n state compared to the rest of the genome.
Please correct me if my assumption is wrong so far.
The question is: A lot of NGS experiments now are enriching for regions (e.g Chip-Seq) how can one calculate the replication time in these experiments? is there a way to normalise for the enrichment variable?
Thank you!
